Journal: Redox Biology

Article Title: Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis

doi: 10.1016/j.redox.2024.103160

Figure Lengend Snippet: List of real time PCR primers and sequences.

Article Snippet: Anti-GLUT2 , Bioss , bs-0351R , WB.

Techniques: Real-time Polymerase Chain Reaction

Journal: Redox Biology

Article Title: Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis

doi: 10.1016/j.redox.2024.103160

Figure Lengend Snippet: List of antibodies used in Western blot analysis and immunofluorescence microscopy.

Article Snippet: Anti-GLUT2 , Bioss , bs-0351R , WB.

Techniques: Western Blot, Immunofluorescence, Microscopy

Journal: Redox Biology

Article Title: Differentiation of intestinal stem cells toward goblet cells under systemic iron overload stress are associated with inhibition of Notch signaling pathway and ferroptosis

doi: 10.1016/j.redox.2024.103160

Figure Lengend Snippet: Iron overload mediated duodenal epithelial function. Iron overload mice model was established by intraperitoneal injection of 120 mg/kg body weight iron dextran once a fortnight for 12 weeks. (A) Representative immunoblot and quantitative analysis showing the expression level of amino acid transporters (BCAT2 and SLC7A5), fatty acid transporter (FABP2) and glucose transporters (SGLT1 and GLUT2) in the duodenum. (B) The mRNA expression levels of BCAT2, SLC7A5, FABP2, SGLT1 and GLUT2 in the duodenum. (C) The mRNA expression levels of secretory factors (CCL9, IL-13, Relm-β, TFF3 and ZG16) in the duodenum. n = 5 for each treatment. * P < 0.05 and ** P < 0.01 compared with the control group.

Article Snippet: Anti-GLUT2 , Bioss , bs-0351R , WB.

Techniques: Injection, Western Blot, Expressing, Control

Journal: Frontiers in Nutrition

Article Title: High-fat diet mouse model receiving L-glucose supplementations propagates liver injury

doi: 10.3389/fnut.2024.1469952

Figure Lengend Snippet: Metabolic profile assessment: (A) Fasting blood sugar (FBS) was assessed as indicated in materials and methods. GLUT2 expressions were quantitated by (B) western blot and (C) RT-PCR methods. (D) Serum insulin C-peptide measured by ELISA. (E) Hepatic expression of inulin receptor (IR) was evaluated from liver sections by western blot (F) HOMA2 calculator calculated HOMA-IR. Paired and unpaired Student’s t -test and ANOVA were used for statistically significant differences. p value was compared between RD and HFD control groups or within RD or HFD groups. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: Rabbit anti-Human/Mouse GYS2 (Proteintech, 22371-1-AP), Rabbit anti-Human/Mouse Glycogen synthase [p Ser641] (Novus bio, NBP2-67315), rabbit anti-human/mouse PYGL antibody (Proteintech, 15851-1-AP), rabbit anti-human/mouse Glut2 polyclonal antibody (Proteintech, 20436-1-AP), rabbit anti-human/mouse ADRP/Perilipin 2 Polyclonal antibody (Proteintech, 15294-1-AP), rabbit anti-human/mouse Alpha Smooth Muscle antibody (Novus, NBP1-30894), mice anti-human/mouse AKT antibody (R&D, MAB 2055), mice anti-human/mouse phospho-AKT antibody (R&D, MAB 887), rabbit anti-human/mouse Insulin Receptor-beta antibody (Proteintech, 20433-1-AP), and rabbit anti-human/mouse beta Actin polyclonal antibody (Proteintech, 20536-1-AP).

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Expressing, Control

Journal: Journal of molecular endocrinology

Article Title: High glucose-induced impairment in insulin secretion is associated with reduction in islet glucokinase in a mouse model of susceptibility to islet dysfunction.

doi: 10.1677/jme.1.01720

Figure Lengend Snippet: Figure 6 (A) Hexokinase and (B) GLUT2 protein levels in DBA/2 (D2, open bars) and C57BL/6 (B6, solid bars) mouse islets incubated with 40 mM glucose for 10 days in the absence or presence of 1 mM aminoguanidine. A liver lysate (L) was used as an internal control. The graph is the mean±S.E.M. of six independent experiments.

Article Snippet: GLUT2 was immunodetected using a specific anti-GLUT2 antibody (cat # sc-9117, Santa Cruz Biotech.

Techniques: Incubation, Control

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Early loss of renal transcripts in kidney allografts: relationship to the development of histologic lesions and alloimmune effector mechanisms.

doi: 10.1111/j.1600-6143.2007.01797.x

Figure Lengend Snippet: Figure 6: Protein expression of se- lected transporters (Slc1a1, Slc2a2). To evaluate if loss of transcripts is as- sociated with loss of protein, we stud- ied protein expression of selected trans- porters by immunohistochemistry at se- lected time points in CBA into B6 allo- grafts. (A) Slc2a2 in normal CBA kidney. (B) Slc2a2 in allografts at day 5. (C) Slc2a2 in allografts at day 7. (D) Slc2a2 in allo- grafts at day 21. (E) Slc1a1 in normal CBA kidney. (F) Slc1a1 in allografts at day 5. (G) Slc1a1 in allografts at day 7. (H) Slc1a1 in allografts at day 21. All pictures were taken at 100x.

Article Snippet: We stained frozen sections (4 lm) with the following antibodies: rat monoclonal to Slc2a2 (anti-Glut2) (R&D Systems); goat polyclonal anti-Slc1a1 (anti-EAAC1) (Chemicon International); HRP-conjugated goat affinity purified F(ab’)2 anti-rat (ICN/Cappel); HRP-conjugated donkey anti-goat IgG (Santa Cruz Biotechnology).

Techniques: Expressing, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs

doi: 10.3390/ijms22084147

Figure Lengend Snippet: A subset of differentially expressed genes in heat stress placentae ( n = 5) compared to control placentae ( n = 5).

Article Snippet: The membranes were then incubated with anti- SLC2A2 rabbit (at 1 μg/mL, Cat# ARP41706_P050, AVIVA Systems Biology, San Diego, CA, USA) and anti- SLC15A1 mouse (1:500 dilution, Cat# sc-373742, Santa Cruz Biotechnology, Dallas, TX, USA) primary antibodies diluted in TBST containing 5% non-fat dry milk overnight at 4 °C.

Techniques: Control, Binding Assay

Journal: International Journal of Molecular Sciences

Article Title: Maternal Heat Stress Alters Expression of Genes Associated with Nutrient Transport Activity and Metabolism in Female Placentae from Mid-Gestating Pigs

doi: 10.3390/ijms22084147

Figure Lengend Snippet: Relative protein expression of glucose transporter 2 (GLUT2; ( A )) and peptide transporter 1 (PEPT1; ( B )) in placentae between control (CON, n = 5) and heat stress (HS, n = 5) groups. Target protein expression was normalised to total protein loaded and expressed as normalised signals (arbitrary units). * p < 0.05. Error bars: standard error of the means.

Article Snippet: The membranes were then incubated with anti- SLC2A2 rabbit (at 1 μg/mL, Cat# ARP41706_P050, AVIVA Systems Biology, San Diego, CA, USA) and anti- SLC15A1 mouse (1:500 dilution, Cat# sc-373742, Santa Cruz Biotechnology, Dallas, TX, USA) primary antibodies diluted in TBST containing 5% non-fat dry milk overnight at 4 °C.

Techniques: Expressing, Control